Titusville is a city and the county seat of Brevard County, Florida, United States. The city’s population was 43,761 as of the 2010 United States Census. Titusville is located along the Indian River, west of Merritt Island and the Kennedy Space Center, and south-southwest of the Canaveral National Seashore. It is a principal city of the Palm BayMelbourne–Titusville Metropolitan Statistical Area.

A secondary, de facto county seat was established beginning in 1989 at Viera, Florida in the geographic center of the county, to better serve the more populous southern portion of the county.